1.無(wú)菌室滅菌。每次使用前開(kāi)啟紫外線(xiàn)燈照射30min以上，或在使用前30min，對內外室用5%石炭酸噴霧。
1. Sterilization in sterile room. Before each use, turn on the ultraviolet lamp for 30min or 30min before use, and use 5% carbolic acid spray in the interior and exterior chambers.
2.用肥皂洗手后，把所需器材搬入外室；在外室換上已滅菌的工作服、工作帽和工作鞋，戴好口罩，然后用2%煤酚皂液將手浸洗2分鐘。
2. After washing hands with soap, move the required equipment into the outer room; Put on sterilized work clothes, hats and shoes in the outer room, wear masks, and then soak your hands with 2% coal phenol soap for 2 minutes.
3.將各種需用物品搬進(jìn)內室清點(diǎn)、就位，用5%石炭酸在工作臺面上方和操作員站位空間噴霧，返回外室，5～10min后再進(jìn)內室工作。
3. move all necessary articles into the inner room for inventory and position. Spray the 5% carbolic acid on the working table and operator's space, return to the outer room, and then enter the inner room after 5 ~ 10min.
4.接種操作前，用70%酒精棉球擦手；進(jìn)行無(wú)菌操作時(shí)，動(dòng)作要輕緩，盡量減少空氣波動(dòng)和地面揚塵。
4. Wipe hands with 70% alcohol cotton ball before inoculation; During aseptic operation, the action shall be gentle to minimize air fluctuation and ground dust.
5.工作中應注意安全。如遇棉塞著(zhù)火，用手緊握或用濕布包裹熄滅，切勿用嘴吹，以免擴大燃燒；如遇有菌培養物灑落或打碎有菌容器時(shí)，應用浸潤5%石炭酸的抹布包裹后，并用浸潤5%石炭酸的抹布擦拭臺面或地面，用酒精棉球擦手后再繼續操作。
5. Pay attention to safety during work. If the tampon catches fire, hold it tightly with your hands or wrap it with a wet cloth. Do not blow it with your mouth to avoid expanding the combustion; In case of bacterial culture spilling or breaking the container, wrap it with a rag soaked with 5% carbolic acid, wipe the table or ground with a rag soaked with 5% carbolic acid, and then continue the operation after wiping your hands with an alcohol cotton ball.
6.工作結束，立即將臺面收拾干凈，將不應在無(wú)菌室存放的物品和廢棄物全部拿出無(wú)菌室后，對無(wú)菌室用5%石炭酸噴霧，或開(kāi)紫外線(xiàn)燈照射30min。
6. at the end of the work, clean up the table immediately, and remove all the articles and wastes that should not be stored in the asepsis room, then use the 5% carbolic acid spray or the ultraviolet lamp to irradiate 30min.
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